Proteomics for Drug Discovery by Iulia M. Lazar Maria Kontoyianni & Alexandru C. Lazar

Author:Iulia M. Lazar, Maria Kontoyianni & Alexandru C. Lazar
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

4.Signal intensity data should be filtered to reduce unreactive proteins, normalize between arrays, and analyze using designated software (e.g., ProteinArray Prospector v.5) (see Notes 21 and 22 ).

4 Notes

1.The PTM profiling protocol is suitable for different human cells . Calibration may be needed to determine the number of cells that yield at least 1 mg protein during functional extract preparation. Recommended protein concentration : 10 mg/mL.

2.When performing cell rupture by repeated freezing and thawing cycles, rapidly thaw the cells in 30 °C and return to liquid nitrogen immediately after thawing.

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